portant; word-wrap: break-word !important; font-family: 宋體;">成分：
portant; word-wrap: break-word !important; font-family: 宋體;"> 成分：
portant; word-wrap: break-word !important; font-family: 宋體;"> 牛肉膏 5g
portant; word-wrap: break-word !important;">portant; word-wrap: break-word !important;">蛋白胨 10g蛋白胨 10g
portant; word-wrap: break-word !important; font-family: 宋體;">氯化鈉 3g
portant; word-wrap: break-word !important; font-family: 宋體;">磷酸氫二鈉 2g
0.2portant; word-wrap: break-word !important; font-family: 宋體;">％滇麝香草酚藍(lán)溶液 12mL
portant; word-wrap: break-word !important; font-family: 宋體;">蒸餾水 1000mL
PH 7.4
portant; word-wrap: break-word !important; font-family: 宋體;">制法
portant; word-wrap: break-word !important; line-height: inherit;">1.portant; word-wrap: break-word !important;"> portant; word-wrap: break-word !important; line-height: inherit; font-family: 宋體;">葡萄糖發(fā)酵管按上述成分配好后portant; word-wrap: break-word !important; line-height: inherit;">,portant; word-wrap: break-word !important; line-height: inherit; font-family: 宋體;">按portant; word-wrap: break-word !important; line-height: inherit;">0.5portant; word-wrap: break-word !important; line-height: inherit; font-family: 宋體;">％加入葡萄糖portant; word-wrap: break-word !important; line-height: inherit;">,portant; word-wrap: break-word !important; line-height: inherit; font-family: 宋體;">分裝于有一個倒置小管的小試管內(nèi)，portant; word-wrap: break-word !important; line-height: inherit;">121portant; word-wrap: break-word !important; line-height: inherit; font-family: 宋體;">℃高壓滅菌portant; word-wrap: break-word !important; line-height: inherit;">15minportant; word-wrap: break-word !important; line-height: inherit; font-family: 宋體;">。
portant; word-wrap: break-word !important; line-height: inherit;">2.portant; word-wrap: break-word !important;"> portant; word-wrap: break-word !important; line-height: inherit; font-family: 宋體;">其他各種糖發(fā)酵管可按上述成分配好后，分裝每瓶portant; word-wrap: break-word !important; line-height: inherit;">1000 mLportant; word-wrap: break-word !important; line-height: inherit; font-family: 宋體;">，portant; word-wrap: break-word !important; line-height: inherit;">121portant; word-wrap: break-word !important; line-height: inherit; font-family: 宋體;">℃高壓滅菌portant; word-wrap: break-word !important; line-height: inherit;">15minportant; word-wrap: break-word !important; line-height: inherit; font-family: 宋體;">。另將各種糖類分別配好portant; word-wrap: break-word !important; line-height: inherit;">10%portant; word-wrap: break-word !important; line-height: inherit; font-family: 宋體;">溶液，同時高壓滅菌。將portant; word-wrap: break-word !important; line-height: inherit;">5mLportant; word-wrap: break-word !important; line-height: inherit; font-family: 宋體;">糖溶液加入于portant; word-wrap: break-word !important; line-height: inherit;">100 mLportant; word-wrap: break-word !important; line-height: inherit; font-family: 宋體;">培養(yǎng)基內(nèi)，以無菌操作分裝小試管。
portant; word-wrap: break-word !important; font-family: 宋體;">注：蔗糖不純，加熱后會自行水解者，應(yīng)采用過濾法除菌。
portant; word-wrap: break-word !important; font-family: 宋體;">試驗方法：portant; word-wrap: break-word !important; font-family: 宋體;">從瓊脂斜面上挑取小量培養(yǎng)物接種，于36portant; word-wrap: break-word !important; font-family: 宋體;">℃±1portant; word-wrap: break-word !important; font-family: 宋體;">℃培養(yǎng)，一般觀察2portant; word-wrap: break-word !important; font-family: 宋體;">～3dportant; word-wrap: break-word !important; font-family: 宋體;">。遲緩反應(yīng)需觀察14~30dportant; word-wrap: break-word !important; font-family: 宋體;">。
portant; word-wrap: break-word !important; color: rgb(0, 128, 255);">portant; word-wrap: break-word !important;">二、乳糖膽鹽發(fā)酵管
portant; word-wrap: break-word !important; font-family: 宋體;">成分：
portant; word-wrap: break-word !important; font-family: 宋體;">蛋白胨 20g
portant; word-wrap: break-word !important; font-family: 宋體;">豬膽鹽（或牛、羊膽鹽）5g
portant; word-wrap: break-word !important; font-family: 宋體;">乳糖 10g
0.04%portant; word-wrap: break-word !important; font-family: 宋體;">滇甲酚紫水溶液 25mL
portant; word-wrap: break-word !important; font-family: 宋體;">蒸餾水 1000mL
portant; word-wrap: break-word !important; font-family: 宋體;">制法：portant; word-wrap: break-word !important; font-family: 宋體;">將蛋白胨，膽鹽及乳糖溶于水中，校正pHportant; word-wrap: break-word !important; font-family: 宋體;">，加入指示劑，分裝每管10 mLportant; word-wrap: break-word !important; font-family: 宋體;">，并放入一個小倒管，115portant; word-wrap: break-word !important; font-family: 宋體;">℃高壓滅菌15minportant; word-wrap: break-word !important; font-family: 宋體;">。
portant; word-wrap: break-word !important; font-family: 宋體;">注：雙料乳糖膽鹽發(fā)酵管除蒸餾水外，其他成分加倍。
portant; word-wrap: break-word !important; font-size: 18px; color: rgb(0, 128, 255);">portant; word-wrap: break-word !important;">三、5portant; word-wrap: break-word !important;">％乳糖發(fā)酵管
portant; word-wrap: break-word !important; font-family: 宋體;">成分：
portant; word-wrap: break-word !important; font-family: 宋體;">蛋白胨 0.2g
portant; word-wrap: break-word !important; font-family: 宋體;">氯化鈉 0.5g
portant; word-wrap: break-word !important; font-family: 宋體;">乳糖 5g
2%portant; word-wrap: break-word !important; font-family: 宋體;">溴麝香草酚藍(lán)水溶液 1.2mL
portant; word-wrap: break-word !important; font-family: 宋體;">蒸餾水 100mL
pH 7.4
portant; word-wrap: break-word !important; font-family: 宋體;">制法：portant; word-wrap: break-word !important; font-family: 宋體;">除乳糖以外的各成分：溶解于50mLportant; word-wrap: break-word !important; font-family: 宋體;">蒸餾水內(nèi)，校正pHportant; word-wrap: break-word !important; font-family: 宋體;">。將乳糖溶解于另外50mLportant; word-wrap: break-word !important; font-family: 宋體;">蒸餾水內(nèi)，分別滅菌121 portant; word-wrap: break-word !important; font-family: 宋體;">℃ 15minportant; word-wrap: break-word !important; font-family: 宋體;">，將兩液混合，以無菌操作分裝于滅菌小試管內(nèi)。
portant; word-wrap: break-word !important; font-family: 宋體;">注：在此培養(yǎng)基內(nèi)，大部分乳糖遲緩發(fā)酵的細(xì)菌可于ldportant; word-wrap: break-word !important; font-family: 宋體;">內(nèi)發(fā)酵。
portant; word-wrap: break-word !important; font-size: 18px;">portant; word-wrap: break-word !important; color: rgb(0, 128, 255);">portant; word-wrap: break-word !important; font-family: 宋體;">四、緩沖葡萄糖蛋白胨水（MRportant; word-wrap: break-word !important; font-family: 宋體;">和VPportant; word-wrap: break-word !important; font-family: 宋體;">試驗用）
portant; word-wrap: break-word !important; font-family: 宋體;">成分portant; word-wrap: break-word !important; font-family: 宋體;">：
portant; word-wrap: break-word !important; font-family: 宋體;">磷酸氫二鉀 5g
portant; word-wrap: break-word !important; font-family: 宋體;">多胨 7g
portant; word-wrap: break-word !important; font-family: 宋體;">葡萄糖 5g
portant; word-wrap: break-word !important; font-family: 宋體;">蒸餾水 1000mL
portant; word-wrap: break-word !important; font-family: 宋體;">制法：portant; word-wrap: break-word !important; font-family: 宋體;">溶化后校正pHportant; word-wrap: break-word !important; font-family: 宋體;">，分裝試管，每管1mL portant; word-wrap: break-word !important; font-family: 宋體;">，121portant; word-wrap: break-word !important; font-family: 宋體;">℃高壓滅菌15minportant; word-wrap: break-word !important; font-family: 宋體;">。
portant; word-wrap: break-word !important; font-family: 宋體;">甲基紅（MRportant; word-wrap: break-word !important; font-family: 宋體;">）試驗：portant; word-wrap: break-word !important; font-family: 宋體;">自瓊脂斜面挑取少量培養(yǎng)物接種本培養(yǎng)基中，于36portant; word-wrap: break-word !important; font-family: 宋體;">±1portant; word-wrap: break-word !important; font-family: 宋體;">℃培養(yǎng)2portant; word-wrap: break-word !important; font-family: 宋體;">～5dportant; word-wrap: break-word !important; font-family: 宋體;">，哈夫尼亞菌則應(yīng)在22portant; word-wrap: break-word !important; font-family: 宋體;">～25portant; word-wrap: break-word !important; font-family: 宋體;">℃培養(yǎng)。滴加甲基紅試劑一滴，立即觀察結(jié)果。鮮紅色為陽性，黃色為陰性，甲基紅試劑配法：10mgportant; word-wrap: break-word !important; font-family: 宋體;">甲基紅溶于30mL95portant; word-wrap: break-word !important; font-family: 宋體;">％乙醇中，然后加入20 mLportant; word-wrap: break-word !important; font-family: 宋體;">蒸餾水。
V-Pportant; word-wrap: break-word !important; font-family: 宋體;">試驗：portant; word-wrap: break-word !important; font-family: 宋體;">用瓊脂培養(yǎng)物接種本培養(yǎng)基中，于36portant; word-wrap: break-word !important; font-family: 宋體;">±1portant; word-wrap: break-word !important; font-family: 宋體;">℃培養(yǎng)2portant; word-wrap: break-word !important; font-family: 宋體;">～4dportant; word-wrap: break-word !important; font-family: 宋體;">。哈夫尼亞菌則應(yīng)在22portant; word-wrap: break-word !important; font-family: 宋體;">～25portant; word-wrap: break-word !important; font-family: 宋體;">℃培養(yǎng)。加入6portant; word-wrap: break-word !important; font-family: 宋體;">％α-portant; word-wrap: break-word !important; font-family: 宋體;">萘酚-portant; word-wrap: break-word !important; font-family: 宋體;">乙醇溶液0.5mLportant; word-wrap: break-word !important; font-family: 宋體;">和40portant; word-wrap: break-word !important; font-family: 宋體;">％氫氧化鉀溶液0.2mLportant; word-wrap: break-word !important; font-family: 宋體;">充分振搖試管，觀察結(jié)果陽性反應(yīng)立刻或于數(shù)分鐘內(nèi)出現(xiàn)紅色，如為陰性，應(yīng)放在36portant; word-wrap: break-word !important; font-family: 宋體;">±1portant; word-wrap: break-word !important; font-family: 宋體;">℃下培養(yǎng)4hportant; word-wrap: break-word !important; font-family: 宋體;">再進(jìn)行觀察。
portant; word-wrap: break-word !important; font-family: 宋體; font-size: 18px; color: rgb(0, 128, 255);">五、營養(yǎng)明膠
portant; word-wrap: break-word !important; font-family: 宋體;">成分：
portant; word-wrap: break-word !important; font-family: 宋體;">蛋白胨 5g
portant; word-wrap: break-word !important; font-family: 宋體;">牛肉膏 3g
portant; word-wrap: break-word !important; font-family: 宋體;">明膠 120g
portant; word-wrap: break-word !important; font-family: 宋體;">蒸餾水 1000mL
pH 6.8~7.0
portant; word-wrap: break-word !important; font-family: 宋體;">制法：portant; word-wrap: break-word !important; font-family: 宋體;">加熱溶解，校正至pH7.4portant; word-wrap: break-word !important; font-family: 宋體;">～7.6portant; word-wrap: break-word !important; font-family: 宋體;">，分裝小管，121portant; word-wrap: break-word !important; font-family: 宋體;">℃高壓滅菌10minportant; word-wrap: break-word !important; font-family: 宋體;">，取出后迅速冷卻，使其凝固，復(fù)查最終pHportant; word-wrap: break-word !important; font-family: 宋體;">應(yīng)為6.8portant; word-wrap: break-word !important; font-family: 宋體;">～7.0portant; word-wrap: break-word !important; font-family: 宋體;">。
portant; word-wrap: break-word !important; font-family: 宋體;">試驗方法：portant; word-wrap: break-word !important; font-family: 宋體;">用瓊脂培養(yǎng)物穿刺接種，放在22portant; word-wrap: break-word !important; font-family: 宋體;">～25portant; word-wrap: break-word !important; font-family: 宋體;">℃培養(yǎng)，每天觀察結(jié)果，記錄液化時間?；蚍旁?6portant; word-wrap: break-word !important; font-family: 宋體;">士1portant; word-wrap: break-word !important; font-family: 宋體;">℃培養(yǎng)，每天取出，放冰箱內(nèi)30minportant; word-wrap: break-word !important; font-family: 宋體;">后再觀察結(jié)果。
portant; word-wrap: break-word !important; font-family: 宋體; font-size: 18px; color: rgb(0, 128, 255);">六、苯丙氨酸培養(yǎng)基
portant; word-wrap: break-word !important; font-family: 宋體;">成分：
portant; word-wrap: break-word !important; font-family: 宋體;">酵母浸膏 3g
DI-portant; word-wrap: break-word !important; font-family: 宋體;">苯丙氨酸（或L-portant; word-wrap: break-word !important; font-family: 宋體;">苯丙氨酸1gportant; word-wrap: break-word !important; font-family: 宋體;">）2g
portant; word-wrap: break-word !important; font-family: 宋體;">磷酸氫二鈉 1g
portant; word-wrap: break-word !important; font-family: 宋體;">氯化鈉 5g
portant; word-wrap: break-word !important; font-family: 宋體;">瓊脂 12g
portant; word-wrap: break-word !important; font-family: 宋體;">蒸餾水 1000mL
portant; word-wrap: break-word !important; font-family: 宋體;">制法：portant; word-wrap: break-word !important; font-family: 宋體;">加熱溶解后分裝試管，121portant; word-wrap: break-word !important; font-family: 宋體;">℃高壓滅菌15minportant; word-wrap: break-word !important; font-family: 宋體;">，使成斜面。
portant; word-wrap: break-word !important; font-family: 宋體;">試驗方法：portant; word-wrap: break-word !important; font-family: 宋體;">自瓊脂斜面上挑取大量培養(yǎng)物，移種于苯丙氨酸瓊脂，在36portant; word-wrap: break-word !important; font-family: 宋體;">士1portant; word-wrap: break-word !important; font-family: 宋體;">℃培養(yǎng)4hportant; word-wrap: break-word !important; font-family: 宋體;">或18portant; word-wrap: break-word !important; font-family: 宋體;">～24hportant; word-wrap: break-word !important; font-family: 宋體;">，滴加10portant; word-wrap: break-word !important; font-family: 宋體;">％三氯化鐵溶液2portant; word-wrap: break-word !important; font-family: 宋體;">～3portant; word-wrap: break-word !important; font-family: 宋體;">滴，自斜面培養(yǎng)物上流下，苯丙氨酸脫氨酶陽性者呈深綠色。
portant; word-wrap: break-word !important; font-family: 宋體; font-size: 18px; color: rgb(0, 128, 255);">七、蛋白胨水（靛基質(zhì)試驗用）
portant; word-wrap: break-word !important; font-family: 宋體;">成分：
portant; word-wrap: break-word !important; font-family: 宋體;">蛋白胨（或胰蛋白胨）20g
portant; word-wrap: break-word !important; font-family: 宋體;">氯化鈉 5g
portant; word-wrap: break-word !important; font-family: 宋體;">蒸餾水 1000mL
pH7.4
portant; word-wrap: break-word !important; font-family: 宋體;">制法：portant; word-wrap: break-word !important; font-family: 宋體;">按上述成分配制，分裝小試管，121portant; word-wrap: break-word !important; font-family: 宋體;">℃高壓滅菌15minportant; word-wrap: break-word !important; font-family: 宋體;">。
portant; word-wrap: break-word !important; font-family: 宋體;">靛基質(zhì)試劑
portant; word-wrap: break-word !important; font-family: 宋體;">柯凡克試劑：將5gportant; word-wrap: break-word !important; font-family: 宋體;">對二甲氨基苯甲醛溶解于75mLportant; word-wrap: break-word !important; font-family: 宋體;">戊醇中，然后緩慢加入濃鹽酸25mlportant; word-wrap: break-word !important; font-family: 宋體;">。
portant; word-wrap: break-word !important; font-family: 宋體;">歐-portant; word-wrap: break-word !important; font-family: 宋體;">波試劑：將1gportant; word-wrap: break-word !important; font-family: 宋體;">對二甲氨基苯甲醛溶解于95mL95%portant; word-wrap: break-word !important; font-family: 宋體;">乙醇內(nèi)，然后緩慢加入濃鹽酸20 mlportant; word-wrap: break-word !important; font-family: 宋體;">。試驗方法：挑取小量培養(yǎng)物接種，在36portant; word-wrap: break-word !important; font-family: 宋體;">士1portant; word-wrap: break-word !important; font-family: 宋體;">℃培養(yǎng)1portant; word-wrap: break-word !important; font-family: 宋體;">～2dportant; word-wrap: break-word !important; font-family: 宋體;">，必要時可培養(yǎng)4portant; word-wrap: break-word !important; font-family: 宋體;">～5dportant; word-wrap: break-word !important; font-family: 宋體;">。加入柯凡克試劑約0.5mLportant; word-wrap: break-word !important; font-family: 宋體;">，輕搖試管，陽性者于試劑層呈深紅色；或加入歐-portant; word-wrap: break-word !important; font-family: 宋體;">波試劑約0.5 mLportant; word-wrap: break-word !important; font-family: 宋體;">，沿管壁流下，覆蓋于培養(yǎng)液表面，陽性者于液面接觸處呈玫瑰紅色。
portant; word-wrap: break-word !important; font-family: 宋體;">注：蛋白胨中應(yīng)含有豐富的色氨酸。每批蛋白胨買來后，應(yīng)先用已知菌種鑒定后方可使用。
portant; word-wrap: break-word !important; font-family: 宋體; font-size: 18px; color: rgb(0, 128, 255);">八、硫酸亞鐵瓊脂（硫化氫試驗用）
portant; word-wrap: break-word !important; font-family: 宋體;">成分：
portant; word-wrap: break-word !important; font-family: 宋體;">牛肉膏 3g
portant; word-wrap: break-word !important; font-family: 宋體;">酵母浸膏 3g
portant; word-wrap: break-word !important; font-family: 宋體;">蛋白胨 10g
portant; word-wrap: break-word !important; font-family: 宋體;">硫酸亞鐵 0.2g
portant; word-wrap: break-word !important; font-family: 宋體;">硫代硫酸鈉 0.3g
portant; word-wrap: break-word !important; font-family: 宋體;">氯化鈉 5g
portant; word-wrap: break-word !important; font-family: 宋體;">瓊脂 12g
portant; word-wrap: break-word !important; font-family: 宋體;">蒸餾水 1000 mL
portant; word-wrap: break-word !important; font-family: 宋體;">制法：portant; word-wrap: break-word !important; font-family: 宋體;">加熱溶解，校正pHportant; word-wrap: break-word !important; font-family: 宋體;">，分裝試管，115portant; word-wrap: break-word !important; font-family: 宋體;">℃高壓滅菌15minportant; word-wrap: break-word !important; font-family: 宋體;">，取出直立候其凝固。
portant; word-wrap: break-word !important; font-family: 宋體;">試驗方法：挑取瓊脂培養(yǎng)物，沿管壁穿刺，于36portant; word-wrap: break-word !important; font-family: 宋體;">士1portant; word-wrap: break-word !important; font-family: 宋體;">℃培養(yǎng)1portant; word-wrap: break-word !important; font-family: 宋體;">～2dportant; word-wrap: break-word !important; font-family: 宋體;">，觀察結(jié)果。產(chǎn)硫化氫者使培養(yǎng)基變?yōu)楹谏?/p>
portant; word-wrap: break-word !important; font-family: 宋體;">注：腸桿菌科細(xì)菌測定硫化氫的產(chǎn)生，應(yīng)采用三糖鐵瓊脂或本培養(yǎng)基。
portant; word-wrap: break-word !important; font-size: 18px; font-family: 宋體; color: rgb(0, 128, 255);">九、尿素瓊脂
portant; word-wrap: break-word !important; font-family: 宋體;">成分：
portant; word-wrap: break-word !important; font-family: 宋體;">蛋白胨 1g
portant; word-wrap: break-word !important; font-family: 宋體;">氯化鈉5gportant; word-wrap: break-word !important; font-family: 宋體;">葡萄糖1g
portant; word-wrap: break-word !important; font-family: 宋體;">磷酸二氫鉀 2g
4%portant; word-wrap: break-word !important; font-family: 宋體;">酚紅溶液 3 mL
portant; word-wrap: break-word !important; font-family: 宋體;">瓊脂 20g
portant; word-wrap: break-word !important; font-family: 宋體;">蒸餾水 1000 mL
20%portant; word-wrap: break-word !important; font-family: 宋體;">尿素溶液 100mL
portant; word-wrap: break-word !important; font-family: 宋體;">制法：portant; word-wrap: break-word !important; font-family: 宋體;">將除尿素和瓊脂以外的成分配好，并校正pHportant; word-wrap: break-word !important; font-family: 宋體;">，加入瓊脂，加熱溶化并分裝燒瓶。121portant; word-wrap: break-word !important; font-family: 宋體;">℃高壓滅菌15minportant; word-wrap: break-word !important; font-family: 宋體;">。冷至50portant; word-wrap: break-word !important; font-family: 宋體;">～55portant; word-wrap: break-word !important; font-family: 宋體;">℃，加入經(jīng)除菌過濾的尿素溶液，尿素的最終濃度為2portant; word-wrap: break-word !important; font-family: 宋體;">％，最終pHportant; word-wrap: break-word !important; font-family: 宋體;">應(yīng)為7.2portant; word-wrap: break-word !important; font-family: 宋體;">±0.1.portant; word-wrap: break-word !important; font-family: 宋體;">分裝于滅菌試管內(nèi)，放成斜面?zhèn)溆谩?/p> portant; word-wrap: break-word !important; font-family: 宋體;">試驗方法：portant; word-wrap: break-word !important; font-family: 宋體;">挑取瓊脂培養(yǎng)物接種，在36portant; word-wrap: break-word !important; font-family: 宋體;">士1portant; word-wrap: break-word !important; font-family: 宋體;">℃培養(yǎng)24hportant; word-wrap: break-word !important; font-family: 宋體;">，觀察結(jié)果。尿素酶陽性者由于產(chǎn)堿而使培養(yǎng)基變?yōu)榧t色。 portant; word-wrap: break-word !important; font-size: 18px; color: rgb(0, 128, 255);">portant; word-wrap: break-word !important;">十、氰化鉀（KCNportant; word-wrap: break-word !important;">）培養(yǎng)基 portant; word-wrap: break-word !important; font-family: 宋體;">成分： portant; word-wrap: break-word !important; font-family: 宋體;">蛋白胨10g portant; word-wrap: break-word !important; font-family: 宋體;">氯化鈉 5g portant; word-wrap: break-word !important; font-family: 宋體;">磷酸二氫鉀 0.225g portant; word-wrap: break-word !important; font-family: 宋體;">磷酸氫二鈉 5.64g portant; word-wrap: break-word !important; font-family: 宋體;">蒸餾水 1000 mL 0.5%portant; word-wrap: break-word !important; font-family: 宋體;">氰化鉀溶液 20 mL pH 7.6 portant; word-wrap: break-word !important; font-family: 宋體;">制法：portant; word-wrap: break-word !important; font-family: 宋體;">將除氰化鉀以外的成分配好后分裝燒瓶，121portant; word-wrap: break-word !important; font-family: 宋體;">℃高壓滅菌15minportant; word-wrap: break-word !important; font-family: 宋體;">。放在冰箱內(nèi)使其充分冷卻。每100 mLportant; word-wrap: break-word !important; font-family: 宋體;">培養(yǎng)基加入0.5portant; word-wrap: break-word !important; font-family: 宋體;">％氰化鉀溶液2.0mLportant; word-wrap: break-word !important; font-family: 宋體;">（最后濃度為1portant; word-wrap: break-word !important; font-family: 宋體;">：10000portant; word-wrap: break-word !important; font-family: 宋體;">），分裝于12mm*100mmportant; word-wrap: break-word !important; font-family: 宋體;">滅菌試管，每管約4mLportant; word-wrap: break-word !important; font-family: 宋體;">，立刻用滅菌橡皮塞塞緊，放在4portant; word-wrap: break-word !important; font-family: 宋體;">℃冰箱內(nèi)，至少可保存兩個月。同時，將不加氰化鉀的培養(yǎng)基作為對照培養(yǎng)基，分裝試管備用。 portant; word-wrap: break-word !important; font-family: 宋體; font-size: 18px; color: rgb(0, 128, 255);">十一、營養(yǎng)瓊脂 portant; word-wrap: break-word !important; font-family: 宋體;">成分： portant; word-wrap: break-word !important; font-family: 宋體;">蛋白胨10g portant; word-wrap: break-word !important; font-family: 宋體;">牛肉膏3g portant; word-wrap: break-word !important; font-family: 宋體;">氯化鈉5g portant; word-wrap: break-word !important; font-family: 宋體;">瓊脂15portant; word-wrap: break-word !important; font-family: 宋體;">～20g portant; word-wrap: break-word !important; font-family: 宋體;">蒸餾水1000mL portant; word-wrap: break-word !important; font-family: 宋體;">制法：portant; word-wrap: break-word !important; font-family: 宋體;">將除瓊脂以外的各成分溶解于蒸餾水內(nèi)，加入15%portant; word-wrap: break-word !important; font-family: 宋體;">氫氧化鈉溶液約2mLportant; word-wrap: break-word !important; font-family: 宋體;">，校正pHportant; word-wrap: break-word !important; font-family: 宋體;">至7.2~7.4portant; word-wrap: break-word !important; font-family: 宋體;">。加入瓊脂，加熱煮沸，使瓊脂溶化分裝燒瓶，121portant; word-wrap: break-word !important; font-family: 宋體;">℃高壓滅菌15minportant; word-wrap: break-word !important; font-family: 宋體;">。 portant; word-wrap: break-word !important; font-family: 宋體;">注：此培養(yǎng)基可供一般細(xì)菌培養(yǎng)之用，可傾注平板或制成斜面。如用于菌落計數(shù)，瓊脂量為1.5%portant; word-wrap: break-word !important; font-family: 宋體;">；如作成平板或斜面，則應(yīng)為2%portant; word-wrap: break-word !important; font-family: 宋體;">。 portant; word-wrap: break-word !important; font-family: 宋體; font-size: 18px; color: rgb(0, 128, 255);">十二、營養(yǎng)肉湯 portant; word-wrap: break-word !important; font-family: 宋體;">成分： portant; word-wrap: break-word !important; font-family: 宋體;">蛋白胨 10g portant; word-wrap: break-word !important; font-family: 宋體;">牛肉膏 3g portant; word-wrap: break-word !important; font-family: 宋體;">氯化鈉 5g portant; word-wrap: break-word !important; font-family: 宋體;">蒸餾水 1000mL pH7.4 portant; word-wrap: break-word !important; font-family: 宋體;">制法：portant; word-wrap: break-word !important; font-family: 宋體;">按上述成分混合，溶解后校正pHportant; word-wrap: break-word !important; font-family: 宋體;">，分裝燒瓶，每瓶225 mL portant; word-wrap: break-word !important; font-family: 宋體;">，121portant; word-wrap: break-word !important; font-family: 宋體;">℃高壓滅菌15minportant; word-wrap: break-word !important; font-family: 宋體;">。 portant; word-wrap: break-word !important; font-size: 18px; color: rgb(0, 128, 255);">portant; word-wrap: break-word !important;">十三、緩沖蛋白胨水（BPportant; word-wrap: break-word !important;">） portant; word-wrap: break-word !important; font-family: 宋體;">成分： portant; word-wrap: break-word !important; font-family: 宋體;">蛋白胨 10g portant; word-wrap: break-word !important; font-family: 宋體;">氯化鈉 5g portant; word-wrap: break-word !important; font-family: 宋體;">磷酸氫二鈉 9g portant; word-wrap: break-word !important; font-family: 宋體;">磷酸二氫鉀 1.5g portant; word-wrap: break-word !important; font-family: 宋體;">蒸餾水1000mL pH 7.2 portant; word-wrap: break-word !important; font-family: 宋體;">制法：portant; word-wrap: break-word !important; font-family: 宋體;">按上述成分配好后以大燒瓶裝，121portant; word-wrap: break-word !important; font-family: 宋體;">℃高壓滅菌15minportant; word-wrap: break-word !important; font-family: 宋體;">。臨用時無菌分裝每瓶225mLportant; word-wrap: break-word !important; font-family: 宋體;">。 portant; word-wrap: break-word !important; font-family: 宋體;">注：本培養(yǎng)基供沙門氏菌前增菌用。 portant; word-wrap: break-word !important; font-family: 宋體;"> portant; word-wrap: break-word !important; font-size: 18px;">portant; word-wrap: break-word !important; color: rgb(0, 128, 255);">portant; word-wrap: break-word !important; font-family: 宋體;">十四、ECportant; word-wrap: break-word !important; font-family: 宋體;">肉湯 portant; word-wrap: break-word !important; font-family: 宋體;">成分： portant; word-wrap: break-word !important; font-family: 宋體;">胰蛋白胨 20g 3portant; word-wrap: break-word !important; font-family: 宋體;">號膽鹽（或混合膽鹽）1.5g portant; word-wrap: break-word !important; font-family: 宋體;">乳糖 5g portant; word-wrap: break-word !important; font-family: 宋體;">磷酸氫二鉀 4g portant; word-wrap: break-word !important; font-family: 宋體;">磷酸二氫鉀 1.5g portant; word-wrap: break-word !important; font-family: 宋體;">氯化鈉 5g portant; word-wrap: break-word !important; font-family: 宋體;">蒸餾水 1000mL portant; word-wrap: break-word !important; font-family: 宋體;">制法：portant; word-wrap: break-word !important; font-family: 宋體;">將上述成分混合，溶解后，分裝有發(fā)酵倒管的試管中，121portant; word-wrap: break-word !important; font-family: 宋體;">℃高壓滅菌15minportant; word-wrap: break-word !important; font-family: 宋體;">。最終pHportant; word-wrap: break-word !important; font-family: 宋體;">為6.9portant; word-wrap: break-word !important; font-family: 宋體;">±0.2portant; word-wrap: break-word !important; font-family: 宋體;">。 portant; word-wrap: break-word !important; font-size: 18px; color: rgb(0, 128, 255);">portant; word-wrap: break-word !important;">十五、四硫磺酸鈉煌綠增菌液（TTBportant; word-wrap: break-word !important;">） portant; word-wrap: break-word !important; font-family: 宋體;">基礎(chǔ)培養(yǎng)基 portant; word-wrap: break-word !important; font-family: 宋體;">多胨或眎胨 5g portant; word-wrap: break-word !important; font-family: 宋體;">膽鹽 1g portant; word-wrap: break-word !important; font-family: 宋體;">碳酸鈣 10g portant; word-wrap: break-word !important; font-family: 宋體;">硫代硫酸鈉 3g portant; word-wrap: break-word !important; font-family: 宋體;">蒸餾水 1000mL portant; word-wrap: break-word !important; font-family: 宋體;">碘溶液 portant; word-wrap: break-word !important; font-family: 宋體;">碘 6g portant; word-wrap: break-word !important; font-family: 宋體;">碘化鉀 5g portant; word-wrap: break-word !important; font-family: 宋體;">蒸餾水 20 mL portant; word-wrap: break-word !important; font-family: 宋體;">制法：portant; word-wrap: break-word !important; font-family: 宋體;">將基礎(chǔ)培養(yǎng)基的各成分加入蒸餾水中，加熱溶解，分裝每瓶100mLportant; word-wrap: break-word !important; font-family: 宋體;">。分裝時應(yīng)隨時振搖，使其中的碳酸鈣混勻，121portant; word-wrap: break-word !important; font-family: 宋體;">℃高壓滅菌15minportant; word-wrap: break-word !important; font-family: 宋體;">備用。臨用時每100mLportant; word-wrap: break-word !important; font-family: 宋體;">基礎(chǔ)培養(yǎng)基中加入碘溶液2mLportant; word-wrap: break-word !important; font-family: 宋體;">，,0.1%portant; word-wrap: break-word !important; font-family: 宋體;">煌綠溶液1mLportant; word-wrap: break-word !important; font-family: 宋體;">。 portant; word-wrap: break-word !important; font-size: 18px; color: rgb(0, 128, 255);">portant; word-wrap: break-word !important;">十六、亞硒酸鹽胱氨酸增菌液（SCportant; word-wrap: break-word !important;">） portant; word-wrap: break-word !important; font-family: 宋體;">成分： portant; word-wrap: break-word !important; font-family: 宋體;">蛋白胨 5g portant; word-wrap: break-word !important; font-family: 宋體;">乳糖 4g portant; word-wrap: break-word !important; font-family: 宋體;">亞硒酸氫鈉 4g portant; word-wrap: break-word !important; font-family: 宋體;">磷酸氫二鈉 5.5g portant; word-wrap: break-word !important; font-family: 宋體;">磷酸二氫鉀 4.5g L-portant; word-wrap: break-word !important; font-family: 宋體;">胱氨酸 0.01g portant; word-wrap: break-word !important; font-family: 宋體;">蒸餾水 1000mL 1% L-portant; word-wrap: break-word !important; font-family: 宋體;">胱氨酸-portant; word-wrap: break-word !important; font-family: 宋體;">氫氧化鈉溶液的配法portant; word-wrap: break-word !important; font-family: 宋體;">：稱取L-portant; word-wrap: break-word !important; font-family: 宋體;">胱氨酸0.1gportant; word-wrap: break-word !important; font-family: 宋體;">或（DL-portant; word-wrap: break-word !important; font-family: 宋體;">胱氨酸0.2gportant; word-wrap: break-word !important; font-family: 宋體;">），加1mol/Lportant; word-wrap: break-word !important; font-family: 宋體;">氫氧化鈉1.5mLportant; word-wrap: break-word !important; font-family: 宋體;">，使溶解，再加入蒸餾水8.5mLportant; word-wrap: break-word !important; font-family: 宋體;">即成。 portant; word-wrap: break-word !important; font-family: 宋體;">制法：portant; word-wrap: break-word !important; font-family: 宋體;">將除亞硒酸氫鈉和L-portant; word-wrap: break-word !important; font-family: 宋體;">胱氨酸以外的各成分溶解于900mLportant; word-wrap: break-word !important; font-family: 宋體;">蒸餾水中，加熱煮沸，候冷備用。另將亞硒酸氫鈉溶解于100mLportant; word-wrap: break-word !important; font-family: 宋體;">蒸餾水中，加熱煮沸，候冷，以無菌操作與上液混合。再加入1% L-portant; word-wrap: break-word !important; font-family: 宋體;">胱氨酸-portant; word-wrap: break-word !important; font-family: 宋體;">氫氧化鈉溶液1mLportant; word-wrap: break-word !important; font-family: 宋體;">。 portant; word-wrap: break-word !important; font-family: 宋體;">分裝于滅菌瓶中．每瓶100mLportant; word-wrap: break-word !important; font-family: 宋體;">，pHportant; word-wrap: break-word !important; font-family: 宋體;">應(yīng)為7.0portant; word-wrap: break-word !important; font-family: 宋體;">±0.1portant; word-wrap: break-word !important; font-family: 宋體;">。 portant; word-wrap: break-word !important; font-size: 18px; color: rgb(0, 128, 255);">portant; word-wrap: break-word !important;">十七、亞硫酸鉍瓊脂（BSportant; word-wrap: break-word !important;">） portant; word-wrap: break-word !important; font-family: 宋體;">成分： portant; word-wrap: break-word !important; font-family: 宋體;">蛋白胨 10g portant; word-wrap: break-word !important; font-family: 宋體;">牛肉膏 5g portant; word-wrap: break-word !important; font-family: 宋體;">葡萄糖 5g portant; word-wrap: break-word !important; font-family: 宋體;">硫酸亞鐵 0.3g portant; word-wrap: break-word !important; font-family: 宋體;">磷酸氫二鈉 4g portant; word-wrap: break-word !important; font-family: 宋體;">煌綠 0.025g portant; word-wrap: break-word !important; font-family: 宋體;">檸檬酸鉍銨 2g portant; word-wrap: break-word !important; font-family: 宋體;">亞硫酸鈉 6g portant; word-wrap: break-word !important; font-family: 宋體;">瓊脂 18~20g portant; word-wrap: break-word !important; font-family: 宋體;">蒸餾水 1000mL pH 7.5 portant; word-wrap: break-word !important; font-family: 宋體;">制法： 1.portant; word-wrap: break-word !important; font-family: 宋體;">將前面5portant; word-wrap: break-word !important; font-family: 宋體;">種成分溶解于300mLportant; word-wrap: break-word !important; font-family: 宋體;">蒸餾水中。 2portant; word-wrap: break-word !important; font-family: 宋體;">將檸檬酸鉍銨和亞硫酸鈉另用50mLportant; word-wrap: break-word !important; font-family: 宋體;">蒸餾水溶解。 3portant; word-wrap: break-word !important; font-family: 宋體;">將瓊脂于600mLportant; word-wrap: break-word !important; font-family: 宋體;">蒸餾水中煮沸溶解，冷至80portant; word-wrap: break-word !important; font-family: 宋體;">℃。 4portant; word-wrap: break-word !important; font-family: 宋體;">將以上三液合并，補(bǔ)充蒸餾水至1000mLportant; word-wrap: break-word !important; font-family: 宋體;">，校正pHportant; word-wrap: break-word !important; font-family: 宋體;">，加0.5%portant; word-wrap: break-word !important; font-family: 宋體;">煌綠水溶液5mLportant; word-wrap: break-word !important; font-family: 宋體;">，搖勻，冷至50portant; word-wrap: break-word !important; font-family: 宋體;">～55portant; word-wrap: break-word !important; font-family: 宋體;">℃，傾注平皿。 portant; word-wrap: break-word !important; font-family: 宋體;">注：此培養(yǎng)基不需高壓滅菌。制備過程不宜過分加熱，以免降低其選擇性。應(yīng)在臨用前一天制備，貯存于室溫暗處，超過48hportant; word-wrap: break-word !important; font-family: 宋體;">不宜使用。 portant; word-wrap: break-word !important; font-size: 18px; color: rgb(0, 128, 255);">portant; word-wrap: break-word !important;">十八、三糖鐵瓊脂（TSIportant; word-wrap: break-word !important;">） portant; word-wrap: break-word !important; font-family: 宋體;">成分： portant; word-wrap: break-word !important; font-family: 宋體;">蛋白胨 20g portant; word-wrap: break-word !important; font-family: 宋體;">牛肉膏 5g portant; word-wrap: break-word !important; font-family: 宋體;">乳糖 10g portant; word-wrap: break-word !important; font-family: 宋體;">蔗糖 10g portant; word-wrap: break-word !important; font-family: 宋體;">葡萄糖 1g portant; word-wrap: break-word !important; font-family: 宋體;">氯化鈉 5g portant; word-wrap: break-word !important; font-family: 宋體;">硫酸亞鐵銨 0.2g portant; word-wrap: break-word !important; font-family: 宋體;">硫代硫酸鈉 0.2g portant; word-wrap: break-word !important; font-family: 宋體;">瓊脂 12g portant; word-wrap: break-word !important; font-family: 宋體;">酚紅 0.025g portant; word-wrap: break-word !important; font-family: 宋體;">蒸餾水 1000 mL pH 7.4portant; word-wrap: break-word !important; line-height: inherit;"> portant; word-wrap: break-word !important; font-family: 宋體;">制法：portant; word-wrap: break-word !important; font-family: 宋體;">將除瓊脂和酚紅以外的各成分溶解于蒸餾水中，校正pHportant; word-wrap: break-word !important; font-family: 宋體;">，加入瓊脂，加熱煮沸，以溶化瓊脂。加入0.2%portant; word-wrap: break-word !important; font-family: 宋體;">酚紅水溶液12.5 mLportant; word-wrap: break-word !important; font-family: 宋體;">，搖勻，分裝試管。裝量宜多些，以便得到較高的底層。121portant; word-wrap: break-word !important; font-family: 宋體;">℃高壓滅菌15minportant; word-wrap: break-word !important; font-family: 宋體;">，放置高層斜面?zhèn)溆谩?/p> portant; word-wrap: break-word !important; font-family: 宋體; font-size: 18px; color: rgb(0, 128, 255);">十九、三糖鐵瓊脂換用方法 portant; word-wrap: break-word !important; font-family: 宋體;">成分： portant; word-wrap: break-word !important; font-family: 宋體;">蛋白胨15g portant; word-wrap: break-word !important; font-family: 宋體;">眎胨5g portant; word-wrap: break-word !important; font-family: 宋體;">牛肉膏 3g portant; word-wrap: break-word !important; font-family: 宋體;">酵母膏 3g portant; word-wrap: break-word !important; font-family: 宋體;">乳糖 10g portant; word-wrap: break-word !important; font-family: 宋體;">蔗糖 10g portant; word-wrap: break-word !important; font-family: 宋體;">葡萄糖 1g portant; word-wrap: break-word !important; font-family: 宋體;">氯化鈉 5g portant; word-wrap: break-word !important; font-family: 宋體;">硫酸亞鐵 0.2g portant; word-wrap: break-word !important; font-family: 宋體;">硫代硫酸鈉 0.3g portant; word-wrap: break-word !important; font-family: 宋體;">瓊脂 12g portant; word-wrap: break-word !important; font-family: 宋體;">酚紅 0.025g portant; word-wrap: break-word !important; font-family: 宋體;">蒸餾水 1000 mL pH7.4 portant; word-wrap: break-word !important; font-family: 宋體;">制法：portant; word-wrap: break-word !important; font-family: 宋體;">將除瓊脂和酚紅以外的各成分溶解于蒸餾水中，校正pHportant; word-wrap: break-word !important; font-family: 宋體;">，加入瓊脂，加熱煮沸，以溶化瓊脂。加入0.2%portant; word-wrap: break-word !important; font-family: 宋體;">酚紅水溶液12.5 mLportant; word-wrap: break-word !important; font-family: 宋體;">，搖勻，分裝試管，裝量宜多些，以便得到較高的底層。121portant; word-wrap: break-word !important; font-family: 宋體;">℃高壓滅菌15minportant; word-wrap: break-word !important; font-family: 宋體;">，放置高層斜面?zhèn)溆谩?/p> portant; word-wrap: break-word !important; font-family: 宋體; font-size: 18px; color: rgb(0, 128, 255);">二十、嗜鹽性試驗培養(yǎng)基 portant; word-wrap: break-word !important; font-family: 宋體;">成分： portant; word-wrap: break-word !important; font-family: 宋體;">蛋白胨2g portant; word-wrap: break-word !important; font-family: 宋體;">氯化鈉 portant; word-wrap: break-word !important; font-family: 宋體;">按不同量加 portant; word-wrap: break-word !important; font-family: 宋體;">蒸餾水100 mL pH7.7 portant; word-wrap: break-word !important; font-family: 宋體;">制法：portant; word-wrap: break-word !important; font-family: 宋體;">配制2%portant; word-wrap: break-word !important; font-family: 宋體;">蛋白胨水，校正pHportant; word-wrap: break-word !important; font-family: 宋體;">，共配制5portant; word-wrap: break-word !important; font-family: 宋體;">瓶，每瓶100mLportant; word-wrap: break-word !important; font-family: 宋體;">。每瓶分別加入不同量的氯化鈉：（1portant; word-wrap: break-word !important; font-family: 宋體;">）不加；（2portant; word-wrap: break-word !important; font-family: 宋體;">）3gportant; word-wrap: break-word !important; font-family: 宋體;">；（3portant; word-wrap: break-word !important; font-family: 宋體;">）7gportant; word-wrap: break-word !important; font-family: 宋體;">；（4portant; word-wrap: break-word !important; font-family: 宋體;">）9gportant; word-wrap: break-word !important; font-family: 宋體;">；（5portant; word-wrap: break-word !important; font-family: 宋體;">）11gportant; word-wrap: break-word !important; font-family: 宋體;">。待溶解后分裝試管，121portant; word-wrap: break-word !important; font-family: 宋體;">℃高壓滅菌15minportant; word-wrap: break-word !important; font-family: 宋體;">。
