南湖新聞網(wǎng)訊（通訊員 宗茂）近日，華中農(nóng)業(yè)大植物科學技術(shù)學院農(nóng)藥毒理學及有害生物抗藥性團隊研究成果以“Redox and Near-Infrared Light-Responsive Nanoplatform for Enhanced Pesticide Delivery and Pest Control in Rice: Construction, Efficacy, and Potential Mechanisms”為題在ACS Applied Materials & Interfaces上發(fā)表。研究采用了一種創(chuàng)新的納米平臺，成功實現(xiàn)了對水稻重要害蟲褐飛虱防控藥劑的高效智能遞送，進一步闡明了其增效機制。
褐飛虱（Nilaparvata lugens）是我國水稻生產(chǎn)上的重要害蟲，對水稻產(chǎn)量和品質(zhì)造成嚴重危害。基于此，研究團隊開發(fā)了一種鑲嵌硫化銅的有機二氧化硅納米復合載體，將其應(yīng)用于新煙堿類殺蟲劑烯啶蟲胺的控制釋放。硫化銅納米顆粒可將近紅外光能轉(zhuǎn)化為熱量，從而實現(xiàn)農(nóng)藥的快速釋放和協(xié)同增效效應(yīng)。同時，有機二氧化硅納米顆粒具有豐富的孔隙結(jié)構(gòu)和較大的比表面積，可高效負載農(nóng)藥分子，并對害蟲體內(nèi)谷胱甘肽作出響應(yīng)。硫化銅可實現(xiàn)快速光熱轉(zhuǎn)化，納米平臺具有較高的光熱轉(zhuǎn)化率，在近紅外光的激發(fā)下，與烯啶蟲胺原藥相比，納米平臺對褐飛虱田間種群和抗藥性種群的毒力均顯著提升。進一步發(fā)現(xiàn)納米平臺可顯著抑制褐飛虱抗藥性種群中P450基因（NlCYP6ER1，NlCYP6AY1，NlCYP4C76）的表達，進而提高其對殺蟲劑的敏感性，研究結(jié)果為害蟲抗藥性的治理提供了新思路。該納米平臺在有害生物綠色高效防控方面具有巨大應(yīng)用潛力。
植物科學技術(shù)學院碩士研究生宗茂為論文第一作者，何順副教授為論文通訊作者，李建洪教授、萬虎教授和馬康生副教授參與了項目的指導。該研究得到湖北省重點研發(fā)計劃和國家自然科學基金等項目資助。
審核人：李建洪
【英文摘要】
The brown planthopper （BPH）， Nilaparvata lugens （St?l）， is a major rice pest in various Asian countries, causing significant negative impacts on rice yield and quality. In this study, we developed a novel nanoplatform （NIT@MON@CuS） for pesticide delivery that responds to redox and near-infrared light stimuli. The nanoplatform consisted of CuS nanoparticles with mesoporous organic silica （MON）， loaded with nitenpyram （NIT）。 With an average size of 190 nm and a loading efficiency of 22%, the NIT@MON@CuS exhibited remarkable thermal response in the near-infrared region, demonstrating excellent photothermal conversion ability and stability. In vitro release kinetics demonstrated that rapid released nitenpyram under near-infrared light and glutathione conditions, facilitating a satisfactory temperature increase and accelerated drug release. The NIT@MON@CuS treated group exhibited a higher mortality of N. lugens, increasing from 62% to 88% compared to the group treated with nitenpyram technical after 96 hours. Bioassay revealed that NIT@MON@CuS significantly enhanced nitenpyram toxicity by more than 1.4-fold against both laboratory insecticide-resistant and field strains of N. lugens. Furthermore, RT-qPCR results demonstrated that MON@CuS had the capability to reduce P450 gene expression thereby improving the sensitivity of N. lugens to insecticides. These findings suggest that MON@CuS hold great potential as an intelligent pest control platform, offering a sustainable and efficient approach to protect crops against pests.
論文鏈接：https://pubs.acs.org/doi/abs/10.1021/acsami.3c08413
日期：2023-10-04
褐飛虱（Nilaparvata lugens）是我國水稻生產(chǎn)上的重要害蟲，對水稻產(chǎn)量和品質(zhì)造成嚴重危害。基于此，研究團隊開發(fā)了一種鑲嵌硫化銅的有機二氧化硅納米復合載體，將其應(yīng)用于新煙堿類殺蟲劑烯啶蟲胺的控制釋放。硫化銅納米顆粒可將近紅外光能轉(zhuǎn)化為熱量，從而實現(xiàn)農(nóng)藥的快速釋放和協(xié)同增效效應(yīng)。同時，有機二氧化硅納米顆粒具有豐富的孔隙結(jié)構(gòu)和較大的比表面積，可高效負載農(nóng)藥分子，并對害蟲體內(nèi)谷胱甘肽作出響應(yīng)。硫化銅可實現(xiàn)快速光熱轉(zhuǎn)化，納米平臺具有較高的光熱轉(zhuǎn)化率，在近紅外光的激發(fā)下，與烯啶蟲胺原藥相比，納米平臺對褐飛虱田間種群和抗藥性種群的毒力均顯著提升。進一步發(fā)現(xiàn)納米平臺可顯著抑制褐飛虱抗藥性種群中P450基因（NlCYP6ER1，NlCYP6AY1，NlCYP4C76）的表達，進而提高其對殺蟲劑的敏感性，研究結(jié)果為害蟲抗藥性的治理提供了新思路。該納米平臺在有害生物綠色高效防控方面具有巨大應(yīng)用潛力。
植物科學技術(shù)學院碩士研究生宗茂為論文第一作者，何順副教授為論文通訊作者，李建洪教授、萬虎教授和馬康生副教授參與了項目的指導。該研究得到湖北省重點研發(fā)計劃和國家自然科學基金等項目資助。
審核人：李建洪
【英文摘要】
The brown planthopper （BPH）， Nilaparvata lugens （St?l）， is a major rice pest in various Asian countries, causing significant negative impacts on rice yield and quality. In this study, we developed a novel nanoplatform （NIT@MON@CuS） for pesticide delivery that responds to redox and near-infrared light stimuli. The nanoplatform consisted of CuS nanoparticles with mesoporous organic silica （MON）， loaded with nitenpyram （NIT）。 With an average size of 190 nm and a loading efficiency of 22%, the NIT@MON@CuS exhibited remarkable thermal response in the near-infrared region, demonstrating excellent photothermal conversion ability and stability. In vitro release kinetics demonstrated that rapid released nitenpyram under near-infrared light and glutathione conditions, facilitating a satisfactory temperature increase and accelerated drug release. The NIT@MON@CuS treated group exhibited a higher mortality of N. lugens, increasing from 62% to 88% compared to the group treated with nitenpyram technical after 96 hours. Bioassay revealed that NIT@MON@CuS significantly enhanced nitenpyram toxicity by more than 1.4-fold against both laboratory insecticide-resistant and field strains of N. lugens. Furthermore, RT-qPCR results demonstrated that MON@CuS had the capability to reduce P450 gene expression thereby improving the sensitivity of N. lugens to insecticides. These findings suggest that MON@CuS hold great potential as an intelligent pest control platform, offering a sustainable and efficient approach to protect crops against pests.
論文鏈接：https://pubs.acs.org/doi/abs/10.1021/acsami.3c08413
日期：2023-10-04
