近日，華中農(nóng)業(yè)大學(xué)油菜團隊研究成果以“Regulation of seed oil accumulation by lncRNAs in Brassica napus”為題在Biotechnology for Biofuels and Bioproducts發(fā)表。研究揭示了lncRNA（long non-coding RNA）可以參與油菜種子油脂合成過程，并探索了lncRNA影響油菜種子油脂積累的潛在機制。該研究結(jié)果不僅豐富了油菜油脂代謝的調(diào)控機制，還為高油油菜籽遺傳改良提供了新線索和理論。
甘藍型油菜是世界上第三大油料作物，約占全球植物油產(chǎn)量的15%。前人的研究已經(jīng)很好地闡明了許多轉(zhuǎn)錄因子和基因參與了種子油脂生物合成的作用機制，但關(guān)于種子油脂合成過程中的調(diào)控研究較少。近年來，lncRNAs（long non-coding RNA）被報道可以通過多種作用機制影響植物的生長、發(fā)育及抗逆等。盡管lncRNA也被預(yù)測可能參與調(diào)控油料作物種子發(fā)育過程中脂質(zhì)代謝過程，但一直缺乏lncRNA影響種子油脂代謝的直接證據(jù)。
該研究通過比較高低含油量油菜種子三個發(fā)育階段lncRNA的表達量變化差異及l(fā)ncRNA-mRNA的共表達變化，鑒定并篩選了一批可能參與油菜種子脂質(zhì)代謝的lncRNAs。對3個候選的lncRNA進行功能驗證，其中有2個lncRNA（MSTRG.86004和MSTRG.22563）可以影響油菜種子含油量的積累。
實驗結(jié)果表明，與WT相比，MSTRG.86004過表達株系的種子含油量增加了約2%，而MSTRG.22563過表達株系減少了3.1-3.9%。進一步研究表明，MSTRG.86004過表達株系種子中，參與脂肪酸合成和種子胚胎發(fā)育的基因（如LEC1）的表達量增加，但參與TAG合成相關(guān)的基因表達量下降。而MSTRG.22563過表達株系中大多數(shù)與脂質(zhì)代謝有關(guān)的基因表達量顯著降低，參與細胞呼吸和三羧酸循環(huán)過程中的相關(guān)代謝產(chǎn)物的含量減少。
以上結(jié)果暗示MSTRG.86004可能在延長種子發(fā)育時間以增加種子含油量方面起作用，而MSTRG.22563可能通過影響種子的光呼吸作用和三羧酸循環(huán)影響種子含油量。
華中農(nóng)業(yè)大學(xué)植物科學(xué)技術(shù)學(xué)院博士后李雨晴為論文第一作者，博士研究生譚增棟負責生物信息學(xué)分析，魯少平副研究員為論文通訊作者，團隊的其他老師及多位研究生參與了此項研究。
【英文摘要】
Background
Studies have indicated that long non-coding RNAs （lncRNAs） play important regulatory roles in many biological processes. However, the regulation of seed oil biosynthesis by lncRNAs remains largely unknown.
Results
We comprehensively identified and characterized the lncRNAs from seeds in three developing stages in two accessions of Brassica napus （B. napus）， ZS11 （high oil content） and WH5557 （low oil content）。 Finally, 8094 expressed lncRNAs were identified. LncRNAs MSTRG.22563 and MSTRG.86004 were predicted to be related to seed oil accumulation. Experimental results show that the seed oil content is decreased by 3.1–3.9% in MSTRG.22563 overexpression plants, while increased about 2% in MSTRG.86004, compared to WT. Further study showed that most genes related to lipid metabolism had much lower expression, and the content of some metabolites in the processes of respiration and TCA （tricarboxylic acid） cycle was reduced in MSTRG.22563 transgenic seeds. The expression of genes involved in fatty acid synthesis and seed embryonic development （e.g., LEC1） was increased, but genes related to TAG assembly was decreased in MSTRG.86004 transgenic seeds.
Conclusion
Our results suggest that MSTRG.22563 might impact seed oil content by affecting the respiration and TCA cycle, while MSTRG.86004 plays a role in prolonging the seed developmental time to increase seed oil accumulation.
論文鏈接：https://doi.org/10.1186/s13068-022-02256-1
日期：2023-05-15
甘藍型油菜是世界上第三大油料作物，約占全球植物油產(chǎn)量的15%。前人的研究已經(jīng)很好地闡明了許多轉(zhuǎn)錄因子和基因參與了種子油脂生物合成的作用機制，但關(guān)于種子油脂合成過程中的調(diào)控研究較少。近年來，lncRNAs（long non-coding RNA）被報道可以通過多種作用機制影響植物的生長、發(fā)育及抗逆等。盡管lncRNA也被預(yù)測可能參與調(diào)控油料作物種子發(fā)育過程中脂質(zhì)代謝過程，但一直缺乏lncRNA影響種子油脂代謝的直接證據(jù)。
該研究通過比較高低含油量油菜種子三個發(fā)育階段lncRNA的表達量變化差異及l(fā)ncRNA-mRNA的共表達變化，鑒定并篩選了一批可能參與油菜種子脂質(zhì)代謝的lncRNAs。對3個候選的lncRNA進行功能驗證，其中有2個lncRNA（MSTRG.86004和MSTRG.22563）可以影響油菜種子含油量的積累。
實驗結(jié)果表明，與WT相比，MSTRG.86004過表達株系的種子含油量增加了約2%，而MSTRG.22563過表達株系減少了3.1-3.9%。進一步研究表明，MSTRG.86004過表達株系種子中，參與脂肪酸合成和種子胚胎發(fā)育的基因（如LEC1）的表達量增加，但參與TAG合成相關(guān)的基因表達量下降。而MSTRG.22563過表達株系中大多數(shù)與脂質(zhì)代謝有關(guān)的基因表達量顯著降低，參與細胞呼吸和三羧酸循環(huán)過程中的相關(guān)代謝產(chǎn)物的含量減少。
以上結(jié)果暗示MSTRG.86004可能在延長種子發(fā)育時間以增加種子含油量方面起作用，而MSTRG.22563可能通過影響種子的光呼吸作用和三羧酸循環(huán)影響種子含油量。
華中農(nóng)業(yè)大學(xué)植物科學(xué)技術(shù)學(xué)院博士后李雨晴為論文第一作者，博士研究生譚增棟負責生物信息學(xué)分析，魯少平副研究員為論文通訊作者，團隊的其他老師及多位研究生參與了此項研究。
【英文摘要】
Background
Studies have indicated that long non-coding RNAs （lncRNAs） play important regulatory roles in many biological processes. However, the regulation of seed oil biosynthesis by lncRNAs remains largely unknown.
Results
We comprehensively identified and characterized the lncRNAs from seeds in three developing stages in two accessions of Brassica napus （B. napus）， ZS11 （high oil content） and WH5557 （low oil content）。 Finally, 8094 expressed lncRNAs were identified. LncRNAs MSTRG.22563 and MSTRG.86004 were predicted to be related to seed oil accumulation. Experimental results show that the seed oil content is decreased by 3.1–3.9% in MSTRG.22563 overexpression plants, while increased about 2% in MSTRG.86004, compared to WT. Further study showed that most genes related to lipid metabolism had much lower expression, and the content of some metabolites in the processes of respiration and TCA （tricarboxylic acid） cycle was reduced in MSTRG.22563 transgenic seeds. The expression of genes involved in fatty acid synthesis and seed embryonic development （e.g., LEC1） was increased, but genes related to TAG assembly was decreased in MSTRG.86004 transgenic seeds.
Conclusion
Our results suggest that MSTRG.22563 might impact seed oil content by affecting the respiration and TCA cycle, while MSTRG.86004 plays a role in prolonging the seed developmental time to increase seed oil accumulation.
論文鏈接：https://doi.org/10.1186/s13068-022-02256-1
日期：2023-05-15
